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Affinity Chromatography Resin
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HIS Protein Purification Resin
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HIS Protein Purification Resin (IDA)
HIS Protein Purification Resin (IDA)
Product Introduction:
- HIS-tag protein purification medium is suitable for the purification of HIS fusion proteins, which can be used to quickly extract His-tag fusion proteins from cell fermentation broth
- HIS protein purification medium (IDA) uses IDA that can tolerate reducing agents as a metal chelating ligand, conventionally chelates Ni2+ (commonly known as nickel filler), and can also chelate Cu2+, Co2+, Zn2+ plasma groups according to customer needs
| Principal Applications | Regulatory Approvals |
| ◦Protein purification (20-500 kDa) | ◦Manufactured under GMP conditions |
| ◦MAb Purification | |
| Advantages | Typical Packaging |
| ◦Manufactured using patented Jetting technique | ◦Bulk Resin |
| ◦Uniform particle size | ◦Production-Scale OPUS® Columns |
| ◦Highest dynamic binding capacity (~80g/l) | ◦OPUS® Robocolumns® |
| ◦Repligen® NGL-ImpactTM A ligand | ◦OPUS® MiniChrom Columns |
| ◦Exceptional alkaline stability | ◦HT Columns |
| ◦Enhanced pressure/flow performance | |
| ◦Manufactured with reduced environmental impact | |
| Parameters | Value |
| Polymer Structure: | Agarose Gel Microspheres |
| Ligand: | IDA |
| Shape: | Sphere |
| Medium average particle size: | 90±5 μm |
| Dynamic binding capacity: | ≥20 mg His-Pro/ml wet gel（IDA） |
| Reducing agent tolerance concentration: | β-mercaptoethanol≤100mM, DTT≤5mM |
| Service life: | 100-150 times (depending on the clarity of the protein solution) |
| Maximum flow rate (25°C): | ≥500 cm/h |
| Maximum Pressure Resistance: | 0.3 MPa (3 bar) |
| Recommended Storage: | 4~8 ℃（20%乙醇） |
