Heparin Affinity Resin
Product Introduction:
- Heparin is an acidic polysaccharide containing sulfate, which can interact with anticoagulant factor III, thrombin, thrombin-like, human coagulation factors IX, XI and VIII, as well as human interleukin, human prostate growth factor, recombinant human vascular endothelial growth factor, soft bone growth factor, basic fibroblast growth factor, recombinant human acidic fibroblast growth factor, recombinant hepatocyte growth factor Recombinant rat heparin cofactor II, recombinant human platelet factor IV, recombinant human endostatin, recombinant human keratinocyte growth factor and other biological macromolecules are combined, so heparin affinity chromatography medium can be used for the purification of these substances.
Application:
- Heparin affinity chromatography medium is widely used for the separation and purification of biological macromolecules such as anticoagulant factor III, thrombin, thrombin-like enzyme, human coagulation factor IX, XI and VIII. Chromatographic operation usually includes column loading, balance, feeding, leaching, elution, regeneration and other steps.
|
Parameters |
Value |
| Base: | 6% cross-linked agarose gel |
| Ligand: | Heparin |
| Column volume: | 5 ml |
| Medium average particle size: | 90±5 μm (50-160) |
| Dynamic binding capacity: | 2mg AT III/ml wet gel |
| Ph stability: | 4~12 (long time); 4~13 (short time) |
| Ligand density: | 3.5mg heparin/ml wet gel |
| Maximum flow rate (25°C): | 300 cm/h |
| Maximum Pressure Resistance: | 0.3 MPa (3 bar) |
| Recommended Storage: | 4~8 ℃（20% ethanol） |
