Home  ꄲ  Bio  ꄲ  Affinity Chromatography Resin  ꄲ  Antibody Purification Resin  ꄲ  Antibody purification medium (ProG)

Antibody purification medium (ProG)

ꄴ Previous： Antibody purification medium (GP ProA)
ꄲNext： Antibody purification medium (ProA)

 

Product Introduction:

  1. Antibody purification medium (ProG) specifically binds to Fc region of the antibody through the conjugated Protein G ligand. After one-step affinity chromatography, high-purity antibodies can be obtained from ascites, serum, culture medium and other samples.
  2. Antibody purification medium (ProG) uses agarose gel as the matrix and recombinant Protein G as the ligand, which has high physical and chemical stability. The ligand is not easy to fall off, has a long life, is convenient to use, and is widely used.

 

Application

  1. Antibody purification medium (ProG) is widely used for the separation and purification of various antibodies. Chromatographic operation usually includes column loading, balance, feeding, leaching, elution, regeneration and other steps.

 

 Principal Applications  Regulatory Approvals
   ◦Protein purification (20-500 kDa)    ◦Manufactured under GMP conditions
   ◦MAb Purification 　
　
 Advantages  Typical Packaging
   ◦Manufactured using patented Jetting technique   ◦Bulk Resin
   ◦Uniform particle size   ◦Production-Scale OPUS® Columns
   ◦Highest dynamic binding capacity (~80g/l)   ◦OPUS® Robocolumns®
   ◦Repligen® NGL-ImpactTM A ligand   ◦OPUS® MiniChrom Columns
   ◦Exceptional alkaline stability   ◦HT Columns
   ◦Enhanced pressure/flow performance 　
   ◦Manufactured with reduced environmental impact 　

 

Parameters Value
 Base: Agarose Gel Microspheres
 Ligand: r-Protein G
 Shape: Sphere
 Medium average particle size: 90 μm (45-165um)
 Dynamic binding capacity: 20-30 mg IgG/ml wet gel
 Ligand density: 5mg Protein A/ml wet gel
 Ph stability: 3~12 (long time); 2~13 (short time)
 Maximum flow rate (25°C): 450 cm/h
 Maximum Pressure Resistance: 0.3 MPa (3 bar)
 Recommended Storage: 4~8 ℃（20%乙醇）